8TY7

Crystal structure of 05.GC.w2.3C10 Fab in complex with H1 HA from A/California/04/2009(H1N1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	I 21 3
Unit cell lengths	201.006, 201.006, 201.006
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.030 - 3.210
R-factor	0.247
Rwork	0.244
R-free	0.28840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m4y
RMSD bond length	0.002
RMSD bond angle	0.438
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.900	3.260
High resolution limit [Å]	3.210	3.210
Rpim	0.044
Number of reflections	22209	2179
<I/σ(I)>	22	0.6
Completeness [%]	100.0	100
Redundancy	22.1
CC(1/2)	0.998	0.230

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M sodium acetate, pH 6.4, 18% PEG3350