8TXL
Nan Regulatory Protein full length mutant R148A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953732 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 62.758, 104.728, 205.659 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.340 - 1.820 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.25240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.136 |
| Data reduction software | XDS (20161205) |
| Data scaling software | Aimless (8.0.013) |
| Phasing software | MOLREP (8.0.012) |
| Refinement software | REFMAC (8.0.013) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.690 | 1.860 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.073 | |
| Number of reflections | 61174 | 3629 |
| <I/σ(I)> | 13.7 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.2 | 11.6 |
| CC(1/2) | 0.999 | 0.628 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293.15 | 0.2 M Lithium sulfate 0.1 M Sodium acetate 4.5 50 % w/v PEG 400 |
