8TX9

Nan Regulatory Protein (core isomerase domain) from Streptococcus pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953649
Spacegroup name	I 4
Unit cell lengths	147.577, 147.577, 82.311
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.230 - 2.010
R-factor	0.2134
Rwork	0.211
R-free	0.27070
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.488
Data reduction software	XDS (20171111)
Data scaling software	Aimless (7.0.076)
Phasing software	PHASER (7.0.076)
Refinement software	REFMAC (8.0.013)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.670	2.060
High resolution limit [Å]	2.010	2.010
Rmerge	0.082
Number of reflections	58739	4202
<I/σ(I)>	15.1
Completeness [%]	99.7
Redundancy	13.5
CC(1/2)	0.999	0.769

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	100mM MES pH 6.5, 12% PEG 20K