8TX8

Crystal Structure of RBBP4 bound to ZNF512B peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95374
Spacegroup name	P 1 21 1
Unit cell lengths	76.103, 59.582, 101.450
Unit cell angles	90.00, 93.77, 90.00

Refinement procedure

Resolution	46.880 - 2.200
R-factor	0.2098
Rwork	0.208
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.543
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.880	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.156	1.238
Rmeas	0.184	1.452
Rpim	0.097	0.754
Number of reflections	46352	6699
<I/σ(I)>	6.5
Completeness [%]	100.0	100
Redundancy	3.5	3.6
CC(1/2)	0.991	0.493

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	Condition Morpheus G2 0.1 M MES/imidazole, pH 6.5, 40% v/v ethylene glycol; 20 % w/v PEG 8000, 0.1 M mixture of carboxylic acids