8TWR

Influenza A virus (A/Aichi/2/1968(H3N2) nucleoprotein mutant - 2-7 deleted, P283S, R416A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-02
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	163.420, 285.220, 116.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.920 - 3.090
R-factor	0.2191
Rwork	0.218
R-free	0.23470
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.920	48.920	3.170
High resolution limit [Å]	3.090	13.830	3.090
Rmerge	0.142	0.041	1.323
Rmeas	0.151	0.044	1.423
Total number of observations	427717
Number of reflections	50131	633	3503
<I/σ(I)>	12.76	38.39	2.14
Completeness [%]	99.6	97.1	95.6
Redundancy	8.532	7.788	7.232
CC(1/2)	0.998	0.999	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M HEPES pH 8.0, 1.01 M potassium sodium tartrate