8TWQ
Structure of bacteriophage lambda RexA protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-06 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 55.649, 55.649, 322.121 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 53.690 - 2.050 |
R-factor | 0.2129 |
Rwork | 0.206 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.038 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1-3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 161.060 | 2.110 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 37768 | 2839 |
<I/σ(I)> | 13.4 | |
Completeness [%] | 100.0 | |
Redundancy | 6.8 | |
CC(1/2) | 0.998 | 0.595 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 2.2 M Ammonium Sulfate 0.2 M Cadmium Chloride |