8TWQ

Structure of bacteriophage lambda RexA protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-06
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	55.649, 55.649, 322.121
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	53.690 - 2.050
R-factor	0.2129
Rwork	0.206
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.038
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1-3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	161.060	2.110
High resolution limit [Å]	2.050	2.050
Number of reflections	37768	2839
<I/σ(I)>	13.4
Completeness [%]	100.0
Redundancy	6.8
CC(1/2)	0.998	0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	2.2 M Ammonium Sulfate 0.2 M Cadmium Chloride