8TWP

Influenza A virus (A/Aichi/2/1968(H3N2) nucleoprotein mutant - 2-7 deleted, R416A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-21
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	C 2 2 21
Unit cell lengths	163.840, 282.920, 116.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.680 - 2.900
R-factor	0.2207
Rwork	0.220
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.557
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.800	2.980
High resolution limit [Å]	2.900	2.900
Number of reflections	59023	4122
<I/σ(I)>	15.71
Completeness [%]	98.1
Redundancy	5.5
CC(1/2)	0.999	0.646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M HEPES pH 8.0, 1.33 % (w/v) polyethylene glycol monomethyl ether 2000, 0.9 M potassium sodium tartrate