8TWO
AvrB bound with UDP and RIN4_T166-Rha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 2.0.1 |
Synchrotron site | ALS |
Beamline | 2.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-06-09 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0358 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 57.990, 119.236, 39.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.150 - 2.090 |
R-factor | 0.2275 |
Rwork | 0.221 |
R-free | 0.28990 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.554 |
Data reduction software | xia2 |
Data scaling software | DIALS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.150 | 2.130 |
High resolution limit [Å] | 2.090 | 2.090 |
Number of reflections | 16986 | 828 |
<I/σ(I)> | 6.61 | |
Completeness [%] | 99.9 | |
Redundancy | 6.3 | |
CC(1/2) | 0.997 | 0.233 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM Tris (pH 7.5~7.8), 27%~32% PEG 550 MME |