8TWD
Structure of IraM-bound RssB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-02 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.977 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 130.664, 191.229, 96.985 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.330 - 3.300 |
R-factor | 0.2474 |
Rwork | 0.245 |
R-free | 0.27060 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.643 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.330 | 3.560 |
High resolution limit [Å] | 3.300 | 3.300 |
Rpim | 0.038 | |
Number of reflections | 18674 | 3782 |
<I/σ(I)> | 17.3 | |
Completeness [%] | 100.0 | |
Redundancy | 13.2 | |
CC(1/2) | 0.790 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1M sodium acetate pH 4.5 120mM magnesium acetate |