8TV8
Crystal structure of nontypeable Haemophilus influenzae SapA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-08 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.978560 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 119.755, 119.755, 129.585 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.014 - 2.250 |
| Rwork | 0.197 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.691 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.014 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.089 | 0.718 |
| Rmeas | 0.091 | 0.737 |
| Rpim | 0.021 | 0.165 |
| Total number of observations | 81561 | |
| Number of reflections | 45307 | 4108 |
| <I/σ(I)> | 16.1 | 4.3 |
| Completeness [%] | 99.9 | |
| Redundancy | 19.2 | 19.9 |
| CC(1/2) | 0.998 | 0.939 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.4 | 295 | 0.1 M Tris pH 9.4, 1.5 M ammonium phosphate, 0.18 M sodium thiocyanate |
