8TV5
Structure of the EphA2 LBDCRD bound to FabS1CE_L1 in a 2:1 (EphA2 to Fab) ratio
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.879, 72.888, 241.962 |
| Unit cell angles | 90.00, 89.04, 90.00 |
Refinement procedure
| Resolution | 120.960 - 4.600 |
| R-factor | 0.2683 |
| Rwork | 0.265 |
| R-free | 0.29820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.899 |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 120.960 | 4.580 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Rmerge | 0.142 | 1.164 |
| Rmeas | 0.168 | 1.393 |
| Rpim | 0.089 | 0.754 |
| Number of reflections | 9743 | 487 |
| <I/σ(I)> | 9.4 | 1 |
| Completeness [%] | 99.0 | 98.2 |
| Redundancy | 3.5 | 3.3 |
| CC(1/2) | 0.997 | 0.495 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 298 | 16% PEG8000, 100 mM CHES 9.0, 200 mM Magnesium Sulfate |
