8TUT
Merkel Cell Polyomavirus LTA NLSct bound to importin alpha 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.95373 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.795, 89.735, 99.526 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.600 - 2.550 |
R-factor | 0.1983 |
Rwork | 0.197 |
R-free | 0.22470 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.598 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.910 | 2.660 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.120 | 0.880 |
Rmeas | 0.140 | 1.000 |
Rpim | 0.060 | 0.450 |
Number of reflections | 23550 | 2824 |
<I/σ(I)> | 7.9 | 2.1 |
Completeness [%] | 99.7 | |
Redundancy | 4.9 | |
CC(1/2) | 0.990 | 0.650 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.70 M sodium citrate, 0.10 M HEPES pH 7.0, 0.01 M DTT |