8TUT

Merkel Cell Polyomavirus LTA NLSct bound to importin alpha 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95373
Spacegroup name	P 21 21 21
Unit cell lengths	78.795, 89.735, 99.526
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.600 - 2.550
R-factor	0.1983
Rwork	0.197
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.598
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.910	2.660
High resolution limit [Å]	2.550	2.550
Rmerge	0.120	0.880
Rmeas	0.140	1.000
Rpim	0.060	0.450
Number of reflections	23550	2824
<I/σ(I)>	7.9	2.1
Completeness [%]	99.7
Redundancy	4.9
CC(1/2)	0.990	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.70 M sodium citrate, 0.10 M HEPES pH 7.0, 0.01 M DTT