8TUR

Merkel Cell Polyomavirus LTA bipartite NLS bound to importin alpha 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95373
Spacegroup name	P 21 21 21
Unit cell lengths	79.035, 89.498, 99.767
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.940 - 2.150
R-factor	0.1823
Rwork	0.181
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.532
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.940	2.220
High resolution limit [Å]	2.150	2.150
Rmerge	0.120	1.290
Rmeas	0.130	1.340
Rpim	0.040	0.380
Number of reflections	39199	3357
<I/σ(I)>	12.8	2.1
Completeness [%]	99.9
Redundancy	11.9
CC(1/2)	1.000	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.65 M sodium citrate, 0.1 M HEPES pH 6.5, 0.01 M DTT