8TUQ

STL Polyomavirus LTA NLS bound to importin alpha 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95373
Spacegroup name	P 21 21 21
Unit cell lengths	78.268, 89.803, 100.655
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.210 - 2.000
R-factor	0.1676
Rwork	0.166
R-free	0.19960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.000
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.210	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.050	0.590
Rmeas	0.060	0.670
Rpim	0.030	0.310
Number of reflections	47684	3523
<I/σ(I)>	15.1	2.9
Completeness [%]	98.4
Redundancy	4.6
CC(1/2)	1.000	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.80 M sodium citrate, 0.1 M HEPES pH 6.5, 0.01 M DTT