8TUH

HLA B7:02 with RPIIRPATL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953651
Spacegroup name	P 61 2 2
Unit cell lengths	57.163, 57.163, 396.369
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.029 - 2.200
R-factor	0.182001570887
Rwork	0.179
R-free	0.24543
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.858
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	2.270
High resolution limit [Å]	2.200	2.200
Rpim	0.064	0.300
Number of reflections	21009	1718
<I/σ(I)>	7.9	2.4
Completeness [%]	100.0
Redundancy	21.1
CC(1/2)	0.993	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG