8TTV

Structure of SNX27 FERM complexed with Fam21A repeat 20 (1289-1302)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953700
Spacegroup name	P 21 21 21
Unit cell lengths	37.965, 74.200, 105.189
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.910 - 2.000
R-factor	0.2124
Rwork	0.207
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.009
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.910	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.240	2.161
Rmeas	0.260	2.366
Rpim	0.101	0.943
Total number of observations	139518	8637
Number of reflections	20806	1385
<I/σ(I)>	6.7	1
Completeness [%]	99.5
Redundancy	6.7	6.2
CC(1/2)	0.989	0.205

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M Sodium Cacodylate pH 6.5 and 22.5% PEG4000