8TTU

Structure of SNX27 FERM complexed with Fam21A repeat 19 (1261 - 1274)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953700
Spacegroup name	P 21 21 21
Unit cell lengths	37.544, 74.497, 102.529
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.250 - 2.360
R-factor	0.2036
Rwork	0.198
R-free	0.25120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.093
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.540	2.450
High resolution limit [Å]	2.360	2.360
Rmerge	0.097	0.545
Rmeas	0.109	0.608
Rpim	0.048	0.263
Total number of observations	64599	6238
Number of reflections	12319	1195
<I/σ(I)>	8.4	1.7
Completeness [%]	98.8
Redundancy	5.2	5.2
CC(1/2)	0.995	0.801

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M Sodium Cacodylate pH 6.5 and 25% PEG3350