8TTT

Structure of SNX27 FERM complexed with Fam21A repeat 15 (1124-1140)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953700
Spacegroup name	P 21 21 21
Unit cell lengths	37.567, 74.371, 105.229
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.950 - 2.350
R-factor	0.1968
Rwork	0.191
R-free	0.25090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.054
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.950	2.410
High resolution limit [Å]	2.330	2.330
Rmerge	0.203	1.524
Rmeas	0.220	1.656
Rpim	0.083	0.632
Total number of observations	88510	5906
Number of reflections	12909	940
<I/σ(I)>	7.9	1.1
Completeness [%]	97.6
Redundancy	6.9	6.3
CC(1/2)	0.995	0.379

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.1 M Sodium Cacodylate pH 6.0 and 22.5% PEG4000