8TTC

Structure of retromer VPS29-VPS35 (483-796) complexed with Fam21A repeat 20 (1289-1302)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-13
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.95365
Spacegroup name	P 43 21 2
Unit cell lengths	88.763, 88.763, 328.291
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.580 - 3.010
R-factor	0.2393
Rwork	0.238
R-free	0.27050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.082
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.900	3.200
High resolution limit [Å]	3.010	3.010
Rmerge	0.137	1.588
Rmeas	0.143	1.652
Rpim	0.040	0.451
Total number of observations	348827	54935
Number of reflections	27047	4201
<I/σ(I)>	11.7	1.3
Completeness [%]	99.6
Redundancy	12.9	13.1
CC(1/2)	0.998	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	293	0.1 M Sodium Citrate pH 4.2, 0.2 M NaCl 16% PEG1000