8TT4
Pseudomonas fluorescens isocyanide hydratase pH=6.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.886 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.750, 56.792, 68.414 |
| Unit cell angles | 90.00, 112.54, 90.00 |
Refinement procedure
| Resolution | 38.520 - 1.200 |
| R-factor | 0.1303 |
| Rwork | 0.129 |
| R-free | 0.15810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.890 |
| Data reduction software | autoXDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.520 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmeas | 0.096 | 1.921 |
| Number of reflections | 123914 | 6174 |
| <I/σ(I)> | 9.8 | 1 |
| Completeness [%] | 99.2 | 97.5 |
| Redundancy | 5.9 | 5.9 |
| CC(1/2) | 0.999 | 0.435 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 23% PEG 3350, 200 mM MgCl2, 2 mM DTT and 100mM sodium citrate pH=6.0 |
