8TT1
Pseudomonas fluorescens isocyanide hydratase pH=5.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.886 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.051, 40.724, 83.430 |
| Unit cell angles | 90.00, 104.42, 90.00 |
Refinement procedure
| Resolution | 32.780 - 1.450 |
| R-factor | 0.1569 |
| Rwork | 0.156 |
| R-free | 0.18180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.746 |
| Data reduction software | autoXDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.340 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmeas | 0.097 | 1.822 |
| Number of reflections | 65010 | 3157 |
| <I/σ(I)> | 10.1 | 1 |
| Completeness [%] | 98.4 | 97.5 |
| Redundancy | 5.5 | 5.2 |
| CC(1/2) | 0.999 | 0.427 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 23% PEG 3350, 200 mM MgCl2, 2 mM DTT and 100mM sodium acetate pH=5.0 |
