8TT0
Pseudomonas fluorescens isocyanide hydratase pH=4.2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.886 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.037, 40.598, 83.283 |
| Unit cell angles | 90.00, 104.28, 90.00 |
Refinement procedure
| Resolution | 40.350 - 1.500 |
| R-factor | 0.1697 |
| Rwork | 0.168 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.771 |
| Data reduction software | autoXDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.350 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmeas | 0.107 | 2.150 |
| Number of reflections | 58834 | 2890 |
| <I/σ(I)> | 9.5 | 0.9 |
| Completeness [%] | 98.9 | 99.2 |
| Redundancy | 5.5 | 5.1 |
| CC(1/2) | 0.999 | 0.404 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | 23% PEG 3350, 200 mM MgCl2, 2 mM DTT, 100mM sodium acetate pH=4.2 |
