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8TSU

Pseudomonas fluorescens G150T-1 isocyanide hydratase at 274 K

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL12-2
Synchrotron siteSSRL
BeamlineBL12-2
Temperature [K]274
Detector technologyPIXEL
Collection date2018-11-28
DetectorDECTRIS PILATUS 6M
Wavelength(s)0.775
Spacegroup nameC 1 2 1
Unit cell lengths72.190, 59.717, 56.295
Unit cell angles90.00, 115.89, 90.00
Refinement procedure
Resolution35.200 - 1.150
R-factor0.1207
Rwork0.120
R-free0.14050
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.007
RMSD bond angle0.880
Data reduction softwareautoXDS
Data scaling softwareAimless
Phasing softwarePHENIX
Refinement softwarePHENIX (1.19.2_4158)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]39.4701.170
High resolution limit [Å]1.1501.150
Rmeas0.0521.720
Number of reflections742063528
<I/σ(I)>120.9
Completeness [%]97.494.3
Redundancy3.83.5
CC(1/2)0.9990.366
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8.629825% PEG 3350, 200 MM MAGNESIUM CHLORIDE, 100MM TRIS-HCL, PH 8.6, 2 MM Dithiothreitol

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