8TSO
KDL bound, nucleotide-free MsbA in open, outward-facing conformation
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.68 |
Software | cryoSPARC |
Symmetry | C2 |
CTF correction | PHASE FLIPPING ONLY |