8TS8
p85alpha/p110alpha heterodimer H1047R mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-21 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.03321 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.221, 120.999, 191.034 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 95.520 - 2.720 |
| R-factor | 0.2268 |
| Rwork | 0.225 |
| R-free | 0.26530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.496 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.520 | 2.870 |
| High resolution limit [Å] | 2.720 | 2.720 |
| Rmerge | 0.095 | |
| Rmeas | 0.103 | |
| Rpim | 0.041 | |
| Number of reflections | 50359 | 7722 |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 93.6 | 100 |
| Redundancy | 6.5 | 7 |
| CC(1/2) | 0.996 | 0.805 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M Hepes pH 7.5, 9% PEG-3350 |
