8TS8

p85alpha/p110alpha heterodimer H1047R mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-21
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.03321
Spacegroup name	P 21 21 21
Unit cell lengths	85.221, 120.999, 191.034
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	95.520 - 2.720
R-factor	0.2268
Rwork	0.225
R-free	0.26530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.496
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.520	2.870
High resolution limit [Å]	2.720	2.720
Rmerge	0.095
Rmeas	0.103
Rpim	0.041
Number of reflections	50359	7722
<I/σ(I)>	12.2
Completeness [%]	93.6	100
Redundancy	6.5	7
CC(1/2)	0.996	0.805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M Hepes pH 7.5, 9% PEG-3350