8TRN
Actin 1 from T. gondii in filaments bound to MgADP and jasplakinolide
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3 |
| Software | PHENIX,cryoSPARC,Leginon,CTFFIND,Coot,UCSF ChimeraX,ISOLDE |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
