8TRI
Crystal Structure of Mouse Cadherin-23 EC25-MAD28 F2894A
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 146.994, 164.657, 183.634 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.563 - 3.716 |
Rwork | 0.187 |
R-free | 0.22940 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.622 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.760 |
High resolution limit [Å] | 3.700 | 3.700 |
Number of reflections | 47174 | 2309 |
<I/σ(I)> | 11.5 | 1.96 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 10.4 | 7 |
CC(1/2) | 0.981 | 0.560 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 277 | 1.25 M Lithium Acetate, 0.1 M MES pH 7.2, 25% glycerol (cryo) |