8TQX

Crystal structure of the Zika virus stem-loop A (SLA) bottom stem

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2022-03-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99
Spacegroup name	P 21 21 21
Unit cell lengths	28.322, 41.947, 97.659
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.830 - 2.090
R-factor	0.2525
Rwork	0.249
R-free	0.27840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.042
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.090
High resolution limit [Å]	2.050	2.050
Rmerge	0.076
Rmeas	0.080
Rpim	0.022	0.484
Number of reflections	7321	314
<I/σ(I)>	23.22	1.42
Completeness [%]	94.4	83.3
Redundancy	10.7	6.7
CC(1/2)	0.989	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	Bis-Tris propane, magnesium chloride (MgCl2), and polyethylene glycol (PEG) 3350