8TQX
Crystal structure of the Zika virus stem-loop A (SLA) bottom stem
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-03-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.322, 41.947, 97.659 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.830 - 2.090 |
| R-factor | 0.2525 |
| Rwork | 0.249 |
| R-free | 0.27840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.042 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.076 | |
| Rmeas | 0.080 | |
| Rpim | 0.022 | 0.484 |
| Number of reflections | 7321 | 314 |
| <I/σ(I)> | 23.22 | 1.42 |
| Completeness [%] | 94.4 | 83.3 |
| Redundancy | 10.7 | 6.7 |
| CC(1/2) | 0.989 | 0.724 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | Bis-Tris propane, magnesium chloride (MgCl2), and polyethylene glycol (PEG) 3350 |
