EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.2 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.1 |
| Software | RELION,SerialEM,CTFFIND |
| Symmetry | C5 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
