8TPK
P6522 crystal form of C. crescentus DriD-ssDNA-DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-01-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 115.094, 115.094, 300.276 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.300 - 3.460 |
R-factor | 0.2634 |
Rwork | 0.259 |
R-free | 0.30270 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.825 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.300 | 3.560 |
High resolution limit [Å] | 3.460 | 3.460 |
Rmerge | 0.137 | 1.480 |
Rpim | 0.038 | 0.408 |
Number of reflections | 14675 | 1434 |
<I/σ(I)> | 9.3 | 1.3 |
Completeness [%] | 92.5 | |
Redundancy | 10.1 | |
CC(1/2) | 0.999 | 0.716 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | 1.5 M ammonium sulphate, 0.1 M Tris pH 7.5 |