8TOZ
Class III PreQ1 riboswitch double mutant U8C/A85G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-1 |
Synchrotron site | SSRL |
Beamline | BL12-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 84.082, 84.082, 280.826 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.350 - 3.000 |
R-factor | 0.2041 |
Rwork | 0.202 |
R-free | 0.22210 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.005 |
RMSD bond angle | 0.694 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.350 | 3.180 |
High resolution limit [Å] | 3.000 | 3.000 |
Rpim | 0.090 | 0.879 |
Number of reflections | 21799 | 1953 |
<I/σ(I)> | 6.5 | 1.5 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 8.5 | 8.7 |
CC(1/2) | 0.976 | 0.961 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 65% Tacsimate pH 7, 50mM Na-CacodylatepH 7, 1mM Spermine HCl, 0.006M Hexammine Cobalt (III)Chloride, 5mM Mg Acetate |