8TOK
nhTMEM16 lipid scramblase in lipid nanodiscs with MSP2N2 scaffold protein in the presence of Ca2+ (open state)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.84 |
| Number of Particle | 1948732 |
| Software | RELION,EPU,Coot,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
