8TN8
Crystal structure of the murine astrovirus capsid spike at 1.75 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 |
Unit cell lengths | 45.328, 48.872, 65.463 |
Unit cell angles | 93.34, 102.91, 114.70 |
Refinement procedure
Resolution | 43.750 - 1.750 |
R-factor | 0.1721 |
Rwork | 0.171 |
R-free | 0.20100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.819 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.760 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 46845 | 2226 |
<I/σ(I)> | 14.4 | 1.9 |
Completeness [%] | 94.8 | 92.52 |
Redundancy | 1.7 | 1.7 |
CC(1/2) | 0.995 | 0.936 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | Potassium formate, PEG 3350 |