8TN7
The Crystal Structure of a human monoclonal antibody (aAb), termed TG10, complexed with a disaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 91.095, 91.095, 202.254 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 62.280 - 1.560 |
| R-factor | 0.17148 |
| Rwork | 0.170 |
| R-free | 0.19939 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.738 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.280 | 1.590 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Number of reflections | 70955 | 4697 |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 99.5 | |
| Redundancy | 14.8 | |
| CC(1/2) | 0.999 | 0.479 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.4M Ammonium Sulfate |
