8TLY

Human ASCC1 Phosphodiesterase/Ligase Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	57.879, 62.105, 136.222
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.730 - 2.800
R-factor	0.2086
Rwork	0.206
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.495
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.730	2.970
High resolution limit [Å]	2.800	2.800
Number of reflections	12679	1990
<I/σ(I)>	9.71
Completeness [%]	99.6
Redundancy	1.72
CC(1/2)	0.988	0.796

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	295	12.5% MPEG 2K, 200 mM imidizole/malate (I/M) pH 5.0, 5% saturated KCl at room temperature. For cryoprotection, 25% ethylene glycol added to the mother liquor