8TLN
STRUCTURAL COMPARISON SUGGESTS THAT THERMOLYSIN AND RELATED NEUTRAL PROTEASES UNDERGO HINGE-BENDING MOTION DURING CATALYSIS
Replaces: 3TLNReplaces: 1TLNReplaces: 2TLNExperimental procedure
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 94.100, 94.100, 131.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.165 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 3.143 |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.600 * |
| Rmerge | 0.163 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.2 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 2.5 (M) | ||
| 2 | 1 | reservoir | calcium acetate | 0.01 (M) | |
| 3 | 1 | reservoir | Tris-HCl | 0.01 (M) | |
| 4 | 1 | reservoir | dimethyl sulfoxide | 7 (%(v/v)) |
