8TL1
Structure of Orthoreovirus RNA Chaperone SigmaNS N17
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 273 |
Detector technology | PIXEL |
Collection date | 2021-05-05 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 77.522, 76.801, 89.090 |
Unit cell angles | 90.00, 94.27, 90.00 |
Refinement procedure
Resolution | 88.840 - 3.160 |
R-factor | 0.21096 |
Rwork | 0.208 |
R-free | 0.25110 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.460 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.840 | 3.200 |
High resolution limit [Å] | 3.160 | 3.200 |
Rmerge | 0.132 | 0.130 |
Rpim | 0.130 | |
Number of reflections | 7841 | 302 |
<I/σ(I)> | 10.09 | |
Completeness [%] | 86.4 | |
Redundancy | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.2% colic acid derivativemix, 0.1 M buffer system 3 (8.5), 30% precipitant Mix3 (Molecular dimensions) |