8TKM

Murine NF-kappaB p50 Rel Homology Region homodimer in complex with 17-mer kappaB DNA from human interleukin-6 (IL-6) promoter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-14
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 65
Unit cell lengths	162.172, 162.172, 60.384
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.430 - 2.800
R-factor	0.2272
Rwork	0.225
R-free	0.27260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nfk
RMSD bond length	0.003
RMSD bond angle	0.616
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.430	2.920
High resolution limit [Å]	2.800	2.800
Number of reflections	22616	2260
<I/σ(I)>	17.9	2
Completeness [%]	99.9
Redundancy	17.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Na-Acetate pH 4.9, 16% (w/v) PEG 1500