8TKL
Murine NF-kappaB p50 Rel Homology Region homodimer in complex with a Test 16-mer kappaB-like DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-14 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.152, 146.452, 67.688 |
| Unit cell angles | 90.00, 98.62, 90.00 |
Refinement procedure
| Resolution | 73.230 - 3.000 |
| R-factor | 0.2276 |
| Rwork | 0.225 |
| R-free | 0.28430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.584 |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 146.450 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.035 | |
| Number of reflections | 17962 | 1805 |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 98.6 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293.15 | 4% Tacsimate pH 6.0, 12% PEG 3350 |
