8TKA
Structure of Orthoreovirus RNA Chaperone SigmaNS R6A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2020-07-28 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 137.111, 137.111, 98.248 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 118.740 - 3.000 |
| R-factor | 0.23349 |
| Rwork | 0.231 |
| R-free | 0.28855 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.579 |
| Data reduction software | DIALS |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 118.740 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.069 | |
| Rpim | 0.023 | |
| Number of reflections | 41323 | 29506 |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 99.6 | |
| Redundancy | 2 | |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.2% colic acid derivative, 0.1M buffer system 3 and 30% precipitant mix 3 (molecular dimension) |
