8TJI
SAM-dependent methyltransferase RedM, apo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9796 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.950, 71.610, 145.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.100 - 1.730 |
R-factor | 0.1685 |
Rwork | 0.167 |
R-free | 0.19920 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.682 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.500 | 1.760 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.065 | 0.241 |
Rmeas | 0.067 | 0.263 |
Rpim | 0.019 | 0.101 |
Number of reflections | 67710 | 3483 |
<I/σ(I)> | 23.6 | 5.8 |
Completeness [%] | 99.4 | 95 |
Redundancy | 11.5 | |
CC(1/2) | 0.999 | 0.960 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2-0.3 M ammonium sulphate, 0.1 M MES pH 6.5, 18-24 % PED MME 5000, 13-16 mg/mL RedM, 1:1 drop ratio, 2-4 uL total |