8TJH
TGP-E, extreme thermostable green fluorescent protein (TGP) with Q66E mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-10-04 |
Detector | RIGAKU HyPix-6000HE |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 38.736, 49.064, 59.108 |
Unit cell angles | 78.55, 72.08, 71.41 |
Refinement procedure
Resolution | 20.420 - 2.000 |
R-factor | 0.172 |
Rwork | 0.169 |
R-free | 0.22870 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.170 |
Data reduction software | CrysalisPro |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.420 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.183 | 0.783 |
Rmeas | 0.223 | |
Rpim | 0.125 | 0.554 |
Number of reflections | 26322 | 1957 |
<I/σ(I)> | 7.6 | 2.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.7 | 5.4 |
CC(1/2) | 0.990 | 0.776 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295.15 | 0.35 M magnesium chloride hexahydrate, 0.1 M Tris hydrochloride pH 8.5, 37.5 % PEG 4000 |