8TJ8

CRYSTAL STRUCTURE OF THE A/Moscow/10/1999(H3N2) INFLUENZA VIRUS HEMAGGLUTININ WITH HUMAN RECEPTOR ANALOG 6'-SLNLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03317
Spacegroup name	H 3 2
Unit cell lengths	100.031, 100.031, 385.290
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.040 - 2.560
R-factor	0.217
Rwork	0.209
R-free	0.25760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.703
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.040	2.640
High resolution limit [Å]	2.550	2.550
Rpim	0.040	0.330
Number of reflections	24519	2399
<I/σ(I)>	17.9	1.6
Completeness [%]	100.0
Redundancy	9.2
CC(1/2)	0.998	0.904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	40% PEG-400 and 0.2 M Calcium acetate, 0.1M HEPES pH 7.5