8TJ6

CRYSTAL STRUCTURE OF THE A/Beijing/353/1989(H3N2) INFLUENZA VIRUS HEMAGGLUTININ WITH HUMAN RECEPTOR ANALOG 6'-SLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	H 3
Unit cell lengths	100.224, 100.224, 689.207
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.930 - 2.850
R-factor	0.2297
Rwork	0.229
R-free	0.27540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.695
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.930	2.950
High resolution limit [Å]	2.850	2.850
Rpim	0.060	0.410
Number of reflections	59464	5953
<I/σ(I)>	12	1.3
Completeness [%]	99.3
Redundancy	6.7
CC(1/2)	0.997	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	1.0 M LiCl and 10% PEG-6000