8THW
Cac1 PIP motif bound to PCNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-08-02 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.615, 87.360, 76.511 |
| Unit cell angles | 90.00, 108.33, 90.00 |
Refinement procedure
| Resolution | 24.890 - 2.600 |
| R-factor | 0.2204 |
| Rwork | 0.216 |
| R-free | 0.26740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5v7k |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.355 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.20.1_4487) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmeas | 0.063 | |
| Number of reflections | 35223 | 1437 |
| <I/σ(I)> | 17.2 | |
| Completeness [%] | 99.2 | 96.9 |
| Redundancy | 4.5 | 2 |
| CC(1/2) | 0.634 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M magnesium acetate tetrahydrate (Mg Ac4H) and 11% w/v PEG3350 |
