8THQ
Nonamer RNA bound to hAgo2-PAZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-19 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 65 |
| Unit cell lengths | 91.936, 91.936, 69.579 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.550 - 2.410 |
| R-factor | 0.21836 |
| Rwork | 0.215 |
| R-free | 0.28369 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.464 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.550 | 2.500 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.091 | 1.026 |
| Rpim | 0.226 | |
| Number of reflections | 13092 | 1301 |
| <I/σ(I)> | 31.5 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.3 | 20.2 |
| CC(1/2) | 0.986 | 0.887 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.1 M HEPES pH 7.0, 30% v/v Jeffamine ED-2001 |
