8TH6
Crystal Structure of the G3BP1 NTF2-like domain bound to USP10 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.390, 78.380, 73.410 |
| Unit cell angles | 90.00, 108.63, 90.00 |
Refinement procedure
| Resolution | 45.200 - 2.340 |
| R-factor | 0.21698 |
| Rwork | 0.213 |
| R-free | 0.29535 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.215 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0349) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.210 | 2.420 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Number of reflections | 15966 | 177 |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 63.1 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.918 | 0.848 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 0.1 M HEPES pH 7.5, 25% PEG3350 |
