8TGX

Crystal structure of C. elegans LGG-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00003
Spacegroup name	P 43 21 2
Unit cell lengths	46.699, 46.699, 127.883
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.870 - 2.620
R-factor	0.2722
Rwork	0.270
R-free	0.32320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.442
Data reduction software	DIALS
Data scaling software	Aimless (0.7.9)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.870	2.715
High resolution limit [Å]	2.620	2.620
Rmerge	0.078	0.225
Number of reflections	4677	446
<I/σ(I)>	21.71
Completeness [%]	99.9
Redundancy	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	0.02 M zinc chloride, 0.1 MES buffer pH 6.0, 14% (w/v) PEG 6000