8TGE
Crystal structure of the Methanosarcina mazei glutamine synthetase in complex with GlnK1
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 137.990, 178.030, 169.048 |
Unit cell angles | 90.00, 90.36, 90.00 |
Refinement procedure
Resolution | 89.020 - 2.300 |
R-factor | 0.3 |
Rwork | 0.165 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.936 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | BALBES |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.020 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rpim | 0.068 | 0.447 |
Number of reflections | 180295 | 13618 |
<I/σ(I)> | 8.3 | 1.8 |
Completeness [%] | 99.6 | |
Redundancy | 3.1 | |
CC(1/2) | 0.994 | 0.631 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 273 | PEG 400, 0.1 M MES pH 6.5 |