8TGB
Crystal structure of root lateral formation protein (RLF) b5-domain from Oryza sativa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-24 |
| Detector | DECTRIS EIGER2 XE 9M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 58.951, 26.138, 82.873 |
| Unit cell angles | 90.00, 95.50, 90.00 |
Refinement procedure
| Resolution | 41.246 - 1.550 |
| R-factor | 0.1762 |
| Rwork | 0.175 |
| R-free | 0.19970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.961 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_3335) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.790 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.071 | 0.666 |
| Rmeas | 0.081 | 0.766 |
| Rpim | 0.039 | 0.371 |
| Total number of observations | 150129 | 7127 |
| Number of reflections | 36463 | 1770 |
| <I/σ(I)> | 11 | 2 |
| Completeness [%] | 97.6 | |
| Redundancy | 4.1 | 4 |
| CC(1/2) | 0.998 | 0.763 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 291 | JCSG+ A6: 20% (w/v) PEG 1000, 100 mM phosphate-citrate pH 4.2, 200 mM lithium sulfate. Cryoprotectant: 80% crystallization solution and 20% (w/v) glycerol. |
